Document Type
Dissertation
Degree
Doctor of Philosophy (PhD)
Major/Program
Biomedical Engineering
First Advisor's Name
Anthony J. McGoron
First Advisor's Committee Title
Committee Chair
Second Advisor's Name
Yen-Chih Huang
Third Advisor's Name
Wei-Chiang Lin
Fourth Advisor's Name
Joong-ho Moon
Keywords
cancer treatment, Nanotechnology
Date of Defense
3-28-2013
Abstract
A novel biocompatible and biodegradable polymer, termed poly(Glycerol malate co-dodecanedioate) (PGMD), was prepared by thermal condensation method and used for fabrication of nanoparticles (NPs). PGMD NPs were prepared using the single oil emulsion technique and loaded with an imaging/hyperthermia agent (IR820) and a chemotherapeutic agent (doxorubicin, DOX). The size of the void PGMD NPs, IR820-PGMD NPs and DOX-IR820-PGMD NPs were approximately 90 nm, 110 nm, and 125 nm respectively. An acidic environment (pH=5.0) induced higher DOX and IR820 release compared to pH=7.4. DOX release was also enhanced by exposure to laser, which increased the temperature to 42°C. Cytotoxicity of DOX-IR820-PGMD NPs was comparable in MES-SA but was higher in Dx5 cells compared to free DOX plus IR820 (pIn vivomouse studies showed that NP formulation significantly improved the plasma half-life of IR820 after tail vein injection. Significant lower IR820 content was observed in kidney in DOX-IR820-PGMD NP treatment as compared to free IR820 treatment in our biodistribution studies (p
Identifier
FI13042501
Recommended Citation
Lei, Tingjun, "Multifunctional Nanoparticles in Cancer: in vitro Characterization, in vivo Distribution" (2013). FIU Electronic Theses and Dissertations. 872.
https://digitalcommons.fiu.edu/etd/872
Included in
Bioimaging and Biomedical Optics Commons, Biomaterials Commons, Biotechnology Commons, Molecular, Cellular, and Tissue Engineering Commons, Nanoscience and Nanotechnology Commons
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