Document Type

Dissertation

Degree

Doctor of Philosophy (PhD)

Major/Program

Biology

First Advisor's Name

Dr. DeEtta Kay Mills

First Advisor's Committee Title

Committee chair

Second Advisor's Name

Dr. Maria Elena Villar

Second Advisor's Committee Title

Committee member

Third Advisor's Name

Dr. Daniel Gann

Third Advisor's Committee Title

Committee member

Fourth Advisor's Name

Dr. Anthony Bellantuono

Fourth Advisor's Committee Title

Committee member

Fifth Advisor's Name

Dr. Matthew DeGennaro

Fifth Advisor's Committee Title

Committee member

Keywords

Citizen Science, Bioinformatics, Aedes aegypti, Mosquito, Whole Genome Sequencing, Pedagogy, Mosquito Surveillance, Population Genomics, Landscape Ecology

Date of Defense

5-17-2023

Abstract

Mosquitoes can transmit deadly vector-borne viruses, causing substantial health risks. To be effective in their abatement, county control plans must understand urban habitats and the community factors that influence vector population growth. The primary objective of this dissertation is to understand mosquito infestation in Miami-Dade County and the extent that citizen science can be utilized for community-based mosquito surveillance programs. Mosquito infestation within Miami-Dade County showed infestation with mosquitoes year-round and a very pronounced difference between spring, summer, and fall (p < 0.001). Patterns in mosquito abundance were associated with meteorological factors: temperature, precipitation, and relative humidity (p < 0.05). A principal component analysis showed three distinct genetic populations separate the South Florida mosquitoes. Most mosquitoes were encompassed in one cluster and included samples from Miami-Dade, Broward, and Manatee County, suggesting the influence of genetic drift and natural selection where there is a long-term population history. Infestation information in this study was gathered through a citizen science program at Florida International University, Florida Aedes Genome Group (FLAGG). Citizen involvement in FLAGG was analyzed to measure the impact of the program on students by comparing them to their peers. The FLAGG students scored significantly better than their peers when surveyed on mosquito abatement and were more likely to be willing to participate in mosquito control surveillance (p < 0.001). Furthermore, FLAGG intern counts and expert counts showed similar seasonal and site-based trends for mosquito abundance (p < 0.001). Programs like FLAGG can be used to improve mosquito control while also generating a benefit to the participant as it fosters a sense of empowerment and engagement.

Identifier

FIDC011202

Files over 15MB may be slow to open. For best results, right-click and select "Save as..."

Share

COinS
 

Rights Statement

Rights Statement

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).