A Link Between Brevetoxin Exposure, Oxidative Stress, and the Unfolded Protein Response in Human Lymphoblast Cells

Authors

Jordan JobsonFollow

Document Type

Thesis

Degree

Master of Science (MS)

Major/Program

Chemistry

First Advisor's Name

Kathleen Rein

First Advisor's Committee Title

Committee chair

Second Advisor's Name

Yuan Liu

Second Advisor's Committee Title

Committee Member

Third Advisor's Name

Alexander Mebel

Third Advisor's Committee Title

Committee Member

Keywords

brevetoxin, oxidative stress, unfolded protein response, human lymphoblast cells, iodoTMT, proteomics, Karenia brevis, proteome, redoxome, endoplasmic reticulum

Date of Defense

10-31-2022

Abstract

As the Florida Red Tide continues to bloom nearly annually in the Gulf of Mexico, marine life and humans alike continue to be adversely affected by exposure to brevetoxins produced by the dinoflagellate Karenia brevis. Generally recognized as neurotoxins, brevotoxins also cause oxidative stress in the marine life exposed at sub-lethal conditions. However, there is little established regarding a clear pathway in which brevetoxins cause oxidative stress or how to alleviate it. Redox proteomics experiments were carried out to get a snapshot of the proteome and redoxome of human lymphoblast cells (GMO2152), with the cells being exposed to PbTx-2 (5 µM) in parallel experiments to PbTx-2 (5 µM) simultaneously with the anti-oxidant MESNA (500 µM). Using iodoTMT tags for sixplex labelling, the proteomics results showed that oxidative stress could be linked to endoplasmic reticulum stress, and the simultaneous treatment with MESNA counteracted the oxidation of PbTx-2 in human lymphoblast cells.

Identifier

FIDC010955

Recommended Citation

Jobson, Jordan, "A Link Between Brevetoxin Exposure, Oxidative Stress, and the Unfolded Protein Response in Human Lymphoblast Cells" (2022). FIU Electronic Theses and Dissertations. 5159.
https://digitalcommons.fiu.edu/etd/5159