The use of capillary electrophoresis (CE) in drug analysis

Authors

Agnes D. Garcia

Document Type

Thesis

Degree

Master of Science (MS)

Major/Program

Forensic Science

First Advisor's Name

Jose R. Almirall

First Advisor's Committee Title

Committee Chair

Second Advisor's Name

Kenneth Furton

Third Advisor's Name

Bruce McCord

Date of Defense

3-28-2005

Abstract

Capillary electrophoresis is currently a very powerful technique for the analysis of seized drugs. A rapid analytical CE method for the screening and quantification of GHB and GBL was achieved using 300mM CTAC/25mM phosphate buffer pH 6.3. Reversed phase HPLC was achieved using 25mM phosphate buffer pH 6.5 and a Cl8 Aqua column. Chiral separation of 9 amphetamine type stimulants was obtained using a highly sulfated gamma-cyclodextrin as a chiral selector. MECC and CZE were compared for the analysis of psilocybin, while a rapid and robust method is presented for the analysis of major opium alkaloids, using dynamically coated capillary columns. The column is coated with a polycation followed by a polyanion coating, using a commercial reagent kit. Using a background electrolyte pH of 2.5 with the addition of hydroxypropyl-beta- cyclodextrin and dimethyl-beta-cyclodextrin, the analysis of morphine, papaverine, codeine, noscapine, and thebaine in opium samples was obtained with great resolution. Finally, separation of common benzodiazedpines was also investigated using CZE and a pH 2.5 phosphate buffer.

Identifier

FI15101517

Recommended Citation

Garcia, Agnes D., "The use of capillary electrophoresis (CE) in drug analysis" (2005). FIU Electronic Theses and Dissertations. 3928.
https://digitalcommons.fiu.edu/etd/3928