Surface plasmon resonance experiments
Document Type
Thesis
Degree
Master of Science (MS)
Major/Program
Physics
First Advisor's Name
Yesim Darici
First Advisor's Committee Title
Committee Chair
Second Advisor's Name
Jiandi Zhang
Third Advisor's Name
Wenzhi Li
Fourth Advisor's Name
Berrin Tansel
Fifth Advisor's Name
Xuewen Wang
Date of Defense
11-20-2007
Abstract
In this study, a Surface Plasmon Resonance (SPR) optical sensor, based on Kretschmann configuration, was designed and constructed. SPR is a useful tool for obtaining quantitative kinetic and affinity information on biomolecular interactions.
The resolution and the sensitivity of our homemade SPR system was determined and optimized by using thiols and sucrose solutions. The data were normalized and processed to increase the resolution of the system. The sensograms were obtained and the kinetic constants were analyzed. The sensogram of the thiols solutions illustrated the process of association of the thiols on gold surface. The sensogram of the sucrose solutions shown, that the sucrose doesn't have association on gold surface.
Identifier
FI15101447
Recommended Citation
Freixas, Angel Luis, "Surface plasmon resonance experiments" (2007). FIU Electronic Theses and Dissertations. 3417.
https://digitalcommons.fiu.edu/etd/3417
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