The development and evaluation of a selective medium for clostridium bifermentans
Document Type
Thesis
Degree
Master of Science (MS)
Major/Program
Please see currently inactive department below.
Major/Program
Medical Laboratory Science
First Advisor's Name
Manoucher Dezfulian
First Advisor's Committee Title
Committee Chair
Second Advisor's Name
Janet Lineback
Third Advisor's Name
Jerry Bash
Date of Defense
11-16-1999
Abstract
A selective agar medium was developed for the isolation of Clostridium bifermentans. The formulation of the C. bifermentans selective agar (CBSA) was a modification of C. botulinum isolation agar (CBI) medium. The modifications included: a) change of the base medium to promote growth of C. bifermentans, b) increase of antimicrobial concentrations to improve selectivity and, c) lowering of the egg yolk concentration to lower the cost. The CBSA medium contains cycloserine (375 µg/ml), sulfamethoxazole (114 µg/ml) and, trimethoprim (6 µg /ml) as selective inhibitory agents. Quantitation of C. bifermentans strains on CBSA yielded counts comparable to those obtained on egg yolk agar control plates. The increased concentration of the selective agents in CBSA suppressed the growth of the lecithinase producing C perfringens, C. sordellii and other fecal organisms tested. Isolation of C. bifermentans from 20 seeded fecal specimens was readily achieved. The CBSA medium permits quantitative recovery of lecithinase positive colonies of C. bifermentans from human feces and can be used for large scale screening of various clinical specimens.
Identifier
FI14062223
Recommended Citation
de Pascale, Biagio A., "The development and evaluation of a selective medium for clostridium bifermentans" (1999). FIU Electronic Theses and Dissertations. 3019.
https://digitalcommons.fiu.edu/etd/3019
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