Employing Limited Next Generation Sequence Data for the Development of Genetic Loci of Phylogenetic and Population Genetic Utility

Authors

Lauren Evenstone, leven001Follow

Document Type

Thesis

Degree

Master of Science (MS)

Major/Program

Forensic Science

First Advisor's Name

Jeffrey Wells

First Advisor's Committee Title

Committe Chair

Second Advisor's Name

Bruce McCord

Second Advisor's Committee Title

Committee Member

Third Advisor's Name

Martin Tracey

Third Advisor's Committee Title

Committee Member

Keywords

Genetic, PCR, Chrysomya, Mitochondrial genome, Next Generation Sequencing, NGS, CLC, genomics, Blowfly

Date of Defense

7-2-2015

Abstract

Massively parallel high throughput sequencers are transforming the scientific research by reducing the cost and time necessary to sequence entire genomes. The goal of this project is to produce preliminary genome assemblies of calliphorid flies using Life Technologies’ Ion Torrent sequencing and Illumina’s MiSeq sequencing. I located, assembled, and annotated a novel mitochondrial genome for one such fly, the little studied Chrysomya pacifica that is central to one hypothesis about blow fly evolution. With sequencing data from Chrysomya megacephala, its forensically relevant sister species, much insight can be gained by alignments, sequence and protein analysis, and many more tools within the CLC Genomics Workbench software program. I present these analyses here of these recently diverged species.

Identifier

FIDC000108

Recommended Citation

Evenstone, Lauren, "Employing Limited Next Generation Sequence Data for the Development of Genetic Loci of Phylogenetic and Population Genetic Utility" (2015). FIU Electronic Theses and Dissertations. 2191.
https://digitalcommons.fiu.edu/etd/2191