Nano-grids of Yeast Cytochrome C
Document Type
Thesis
Degree
Master of Science (MS)
Major/Program
Biomedical Engineering
First Advisor's Name
Richard T. Schoephoerster
First Advisor's Committee Title
Committee Chair
Second Advisor's Name
Malek Adjouadi
Third Advisor's Name
Sakhrat Khizroev
Fourth Advisor's Name
Watson Lees
Date of Defense
3-31-2005
Abstract
One innovative thought in biomolecular electronics is the exploitation of electron transfer proteins. Using nature's self assembly techniques, proteins can build highly organized edifices with retained functional activity, and they can serve as platforms for biosensors.
In this research work, Yeast Cytochrome C (YCC) is immobilized with a help of a linker molecule, 3-Mercaptopropyltrimethoxysilane (3-MPTS) on a hydroxylated surface of a silicon substrate. Atomic Force Microscopy (AFM) is used for characterization. AFM data shows immobilization of one YCC molecule in between eight grids that are formed by the linker molecules. 3-MPTS monolayers are organized in grids that are 1.2 nm apart. Immobilization of 3-MPTS was optimized using a concentration of 5 mM in a completely dehydrated state for 30 minutes.
The functionally active grids of YCC can now be incorporated with Cytochrome C oxidase on a Platinum electrode surface for transfer of electrons in development of biosensors, such as nitrate sensor, that are small in size, cheaper, and easier to manufacture than the top-down approach of fabrication of molecular biodevices
Identifier
FI14032345
Recommended Citation
Arjomandi, Human, "Nano-grids of Yeast Cytochrome C" (2005). FIU Electronic Theses and Dissertations. 1312.
https://digitalcommons.fiu.edu/etd/1312
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