Secondary Ion Mass Spectrometry Imaging of Dictyostelium discoideum Aggregation Streams

Authors

John Daniel DeBord, Department of Chemistry and Biochemistry, Florida International UniversityDepartment ofFollow
Donald F. Smith, FOM Institute AMOLF
Christopher R. Anderton, Pacific Northwest National Laboratory
Ron M.A. Heeren, FOM Institute AMOLF
Ljiljana Paša-Tolić, Pacific Northwest Laboratory
Richard H. Gomer, Texas A & M University - College Station
Francisco Fernandez-Lima, Department of Chemistry and Biochemistry, Florida International UniversityFollow

Date of this Version

6-9-2014

Document Type

Article

Abstract

High resolution imaging mass spectrometry could become a valuable tool for cell and developmental biology, but both, high spatial and mass spectral resolution are needed to enable this. In this report, we employed Bi₃ bombardment time-of-flight (Bi₃ ToF-SIMS) and C₆₀ bombardment Fourier transform ion cyclotron resonance secondary ion mass spectrometry (C₆₀ FTICR-SIMS) to image Dictyostelium discoideum aggregation streams. Nearly 300 lipid species were identified from the aggregation streams. High resolution mass spectrometry imaging (FTICR-SIMS) enabled the generation of multiple molecular ion maps at the nominal mass level and provided good coverage for fatty acyls, prenol lipids, and sterol lipids. The comparison of Bi₃ ToF-SIMS and C₆₀ FTICR-SIMS suggested that while the first provides fast, high spatial resolution molecular ion images, the chemical complexity of biological samples warrants the use of high resolution analyzers for accurate ion identification.

Comments

This article was originally published in PLoS One.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

Recommended Citation

DeBord JD, Smith DF, Anderton CR, Heeren RMA, Paša-Tolić L, Gomer RH, et al. (2014) Secondary Ion Mass Spectrometry Imaging of Dictyostelium discoideum Aggregation Streams. PLoS ONE 9(6): e99319. doi:10.1371/journal.pone.0099319