A Novel Insight into Dehydroleucodine Mediated Attenuation of Pseudomonas aeruginosa Virulence Mechanism

Authors

Sushmita Mustafi, Department of Biological Sciences, Florida International UniversityFollow
Maria Luisa Veisaga, Biomolecular Sciences Institute, Florida International UniversityFollow
L. A. Lopez, National University of CuyoFollow
Manuel A. Barbieri, Department of Biological Sciences, Biomolecular Institute and International Center of Tropical Botany, Florida International University; Fairchild Tropical Botanic GardenFollow

Date of this Version

11-16-2015

Document Type

Article

Abstract

Increasing resistance of Pseudomonas aeruginosa (P. aeruginosa) to conventional treatments demands the search for novel therapeutic strategies. In this study, the antimicrobial activity of dehydroleucodine (DhL), a sesquiterpene lactone obtained from Artemisia (A.) douglasiana, was screened against several pathogenic virulence effectors of P. aeruginosa. In vitro, minimum inhibitory concentration of DhL was determined against P. aeruginosa strains PAO1, PA103, PA14, and multidrug resistant clinical strain, CDN118. Results showed that DhL was active against each strain where PAO1 and PA103 showed higher susceptibility (MIC 0.48 mg/mL) as compared to PA14 (MIC 0.96 mg/mL) and CDN118 (MIC 0.98 mg/mL). Also, when PAO1 strain was grown in the presence of DhL (MIC50, 0.12 mg/mL), a delay in the generation time was noticed along with significant inhibition of secretory protease and elastase activities, interruption in biofilm attachment phase in a stationary culture, and a significant decline in Type III effector ExoS. At MIC50, DhL treatment increased the sensitivity of P. aeruginosa towards potent antibiotics. Furthermore, treatment of P. aeruginosa with DhL prevented toxin-induced apoptosis in macrophages. These observations suggest that DhL activity was at the bacterial transcriptional level. Hence, antimicrobial activity of DhL may serve as leads in the development of new anti-Pseudomonas pharmaceuticals.

Comments

Originally published in BioMed Research International.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

Recommended Citation

Mustafi, Sushmita; Veisaga, Maria Luisa; Lopez, L. A.; and Barbieri, Manuel A., "A Novel Insight into Dehydroleucodine Mediated Attenuation of Pseudomonas aeruginosa Virulence Mechanism" (2015). Department of Biological Sciences. 97.
https://digitalcommons.fiu.edu/cas_bio/97