Inhibition of Zn(II) binding type IA topoisomerases by organomercury compounds and Hg(II)

Authors

Bokun Cheng, Department of Biochemistry and Molecular Biology, New York Medical College
Thirunavukkarasu Annamalai, Department of Chemistry and Biochemistry, Florida International UniversityFollow
Shayna Sandhaus, Department of Chemistry and Biochemistry, Florida International UniversityFollow
Priyanka Bansod, Department of Chemistry and Biochemistry, Florida International UniversityFollow
Yuk-Ching Tse-Dinh, Department of Chemistry and Biochemistryand Biomolecular Sciences Institute, Florida International UniversityFollow

Date of this Version

1-1-2015

Document Type

Article

Rights

default

Abstract

Type IA topoisomerase activities are essential for resolving DNA topological barriers via an enzyme-mediated transient single strand DNA break. Accumulation of topoisomerase DNA cleavage product can lead to cell death or genomic rearrangement. Many antibacterial and anticancer drugs act as topoisomerase poison inhibitors that form stabilized ternary complexes with the topoisomerase covalent intermediate, so it is desirable to identify such inhibitors for type IA topoisomerases. Here we report that organomercury compounds were identified during a fluorescence based screening of the NIH diversity set of small molecules for topoisomerase inhibitors that can increase the DNA cleavage product of Yersinia pestis topoisomerase I. Inhibition of relaxation activity and accumulation of DNA cleavage product were confirmed for these organomercury compounds in gel based assays of Escherichia coli topoisomerase I. Hg(II), but not As(III), could also target the cysteines that form the multiple Zn(II) binding tetra-cysteine motifs found in the C-terminal domains of these bacterial topoisomerase I for relaxation activity inhibition. Mycobacterium tuberculosis topoisomerase I activity is not sensitive to Hg(II) or the organomercury compounds due to the absence of the Zn(II) binding cysteines. It is significant that the type IA topoisomerases with Zn(II) binding domains can still cleave DNA when interfered by Hg(II) or organomercury compounds. The Zn(II) binding domains found in human Top3α and Top3β may be potential targets of toxic metals and organometallic complexes, with potential consequence on genomic stability and development.

DOI

10.1371/journal.pone.0120022

Identifier

25798600

Comments

Copyright: © 2015 Cheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

Recommended Citation

Cheng B, Annamalai T, Sandhaus S, Bansod P, Tse-Dinh Y-C (2015) Inhibition of Zn(II) Binding Type IA Topoisomerases by Organomercury Compounds and Hg(II). PLoS ONE 10(3): e0120022. https://doi.org/10.1371/journal.pone.0120022