A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons

Authors

Thirunavukkarasu Annamalai, Department of Chemistry and Biochemistry, Florida International UniversityFollow
Bokun Cheng, Department of Pediatrics, Albert Einstein College of Medicine
Neelam Keswani, Department of Chemistry and Biochemistry, Florida International UniversityFollow
Yuk-Ching Tse-Dinh, Department of Chemistry and Biochemistry, Florida International UniversityFollow

Date of this Version

1-1-2018

Document Type

Article

Rights

default

Abstract

Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.

DOI

10.1007/978-1-4939-7459-7_18

Identifier

29177747

Recommended Citation

Annamalai T., Cheng B., Keswani N., Tse-Dinh YC. (2018) A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons. In: Drolet M. (eds) DNA Topoisomerases. Methods in Molecular Biology, vol 1703. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7459-7_18