Fast, ultra-trace detection of juvenile hormone III from mosquitoes using mass spectrometry

Authors

Cesar E. Ramirez, Department of Chemistry and Biochemistry, Florida International UniversityFollow
Marcela Nouzova, Department of Biological Sciences and Biomolecular Sciences Institute, Florida International UniversityFollow
Paolo Benigni, Department of Chemistry and Biochemistry, Florida International UniversityFollow
Martin Quirke, Department of Chemistry and Biochemistry, Florida International UniversityFollow
Fernando G. Noriega, Department of Biological Sciences and Biomolecular Sciences Institute, Florida International UniversityFollow
Francisco Fernandez-Lima, Biomolecular Sciences Institute and Department of Chemistry and Biochemistry, Florida International UniversityFollow

Date of this Version

2016

Document Type

Article

Rights

default

Abstract

In the present work, a new protocol for fast separation and quantification of JH III from biological samples using liquid chromatography coupled to electrospray tandem mass spectrometry is described. In particular, the proposed protocol improves existing methodologies by combining a limited number of sample preparation steps with fast LC-MS/MS detection, providing lower limits of detection and demonstrated matrix effect control, together with high inter and intraday reproducibility. A limit of detection of 8 pg/mL (0.32 pg on column) was achieved, representing a 15-fold gain in sensitivity with respect to previous LC-MS based protocols. The performance of the LC-MS/MS protocol is comparable to previously described JH III quantitation protocol based on fluorescence detection, with the added advantage that quantification is independent of the availability of fluorescent tags that are often unavailable or show quite diverse responses on a batch-to-batch basis. Additionally, a detailed description of the JH III fragmentation pathway is provided for the first time, based on isolation of the molecular ion and their intermediate fragments using in-source MS/MS, MS/MSⁿ and FT-ICR MS/MS measurements. The JH III workflow was evaluated as a function of developmental changes, sugar feeding and farnesoic acid stimulation in mosquitoes and can be applied to the detection of other juvenile hormones.

DOI

10.1016/j.talanta.2016.06.041

Identifier

FIDC001717

Comments

Post Print Version.

The definitive publisher-authenticated version is available online at https://doi.org/10.1016/j.talanta.2016.06.041

Recommended Citation

Ramirez, Cesar E.; Nouzova, Marcela; Benigni, Paolo; Quirke, Martin; Noriega, Fernando G.; and Fernandez-Lima, Francisco, "Fast, ultra-trace detection of juvenile hormone III from mosquitoes using mass spectrometry" (2016). Biomolecular Sciences Institute: Faculty Publications. 26.
https://digitalcommons.fiu.edu/biomolecular_fac/26