Date of this Version

8-4-2015

Document Type

Article

Abstract

8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a typical biomarker of oxidative DNA damage and has attracted much attention in recent years since the level of 8-OHdG in body fluids is typically associated with various diseases. In this work, a simple and highly sensitive electrochemical sensor for the determination of 8-OHdG was fabricated incorporating single wall carbon nanotubes-(SWCNTs-) Nafion composite film coated on glassy carbon electrode. Nafion was chosen as an optimal adhesive agent from a series of adhesive agents and acted as a binder, enrichment, and exclusion film. Due to the strong cation-exchange ability of Nafion and the outstanding electronic properties ofSWCNTs, the prepared SWCNTs-Nafion film can strongly enhance the electrochemical response to oxidation of 8-OHdG and efficiently alleviate the interferences from uric acid and ascorbic acid. The oxidation peak currents are linear with the concentration of 8-OHdG in the range of 0.03 to 1.25 mu M with a detection limit of 8.0 nM (S/N = 3). This work demonstrates that SWCNTs-Nafion film can improve the sensitivity, selectivity, reproducibility, and stability, making it an ideal candidate for electrochemical detection of 8-OHdG.

Comments

Originally published in the Journal of Sensors.

Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

Download

Share

COinS
 

Rights Statement

Rights Statement

In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).