Characterization of LuxA of novel strains of the genus Shewanella
Master of Science (MS)
First Advisor's Name
John C. Makemson
First Advisor's Committee Title
Second Advisor's Name
David N. Kuhn
Third Advisor's Name
Luminous bacteria, Shewanella
Date of Defense
Bioluminescence is a trait observed among different genera and families of bacteria. In this study part of the luxA gene was characterized from the new MS isolates and compared to luxA of other bacteria. The polymerase chain reaction (PCR) was used to amplify a fragment of the luxA gene of strain MS32 and Vibrio harveyi. These fragments were used as probes in hybridization experiments with luminous and nonluminous bacteria. The results from these experiments suggest that some nonluminous species may possess lux like regions in their chromosomal DNA and that luxA probes can demonstrate species identity. The MS32 luxA fragment was also sequenced and used in a phylogenetic analysis to identify the taxonomic affinities of MS strains. It was found that MS1 and MS32 were closely related, however, Shewanella hanedai was not. Thus there was a concordance between the phenotypic and genotypic approaches, which will help in establishing a consistent taxonomic affinity between these bacteria.
Fulayfil, Nada Rashid, "Characterization of LuxA of novel strains of the genus Shewanella" (1994). FIU Electronic Theses and Dissertations. 3425.
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