Document Type



Master of Science (MS)


Biomedical Engineering

First Advisor's Name

Vekalet Tek

First Advisor's Committee Title

Committee Chair

Second Advisor's Name

Wei-Chiang Lin

Third Advisor's Name

Leonel Lagos

Fourth Advisor's Name

Nezih Pala

Date of Defense



The objective of this study is to design and development of an enzyme-linked biosensor for detection and quantification of phosphate species. Various concentrations of phosphate species were tested and completed for this study.

Phosphate is one of the vital nutrients for all living organisms. Phosphate compounds can be found in nature (e.g., water sediments), and they often exist in aninorganic form. The amount of phosphates in the environment strongly influences the operations of living organisms. Excess amount of phosphate in the environment causes eutrophication which in turn causes oxygen deficit for the other living organisms. Fish die and degradation of habitat in the water occurs as a result of eutrophication. In contrast, low phosphate concentration causes death of vegetation since plants utilize the inorganic phosphate for photosynthesis, respiration, and regulation of enzymes. Therefore, the phosphate quantity in lakes and rivers must be monitored.

Result demonstrated that phosphate species could be detected in various organisms via enzyme-linked biosensor in this research.




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