Document Type
Thesis
Degree
Master of Science (MS)
Major/Program
Forensic Science
First Advisor's Name
Jose Almirall
First Advisor's Committee Title
Committee Chair
Second Advisor's Name
Alan Meerow
Third Advisor's Name
Raymond Schnell
Fourth Advisor's Name
Kenneth Furton
Date of Defense
1-9-2003
Abstract
Microsatellite markers were developed for Cannabis sativa L. (marijuana) to estimate the level of polymorphism, usefulness for DNA typing (genotype identification), and to measure the genetic relationships between the different plants. Twelve different oligonucleotide probes were used to screen an enriched microsatellite library of Cannabis sativa in which 49% of the clones contained microsatellite sequences. Characterization of microsatellite loci in Cannabis revealed that GA/CT was the most abundant class of isolated microsatellites representing 50% overall. Eleven polymorphic SSR markers were developed, derived from dinucleotide motifs and eight from trinucleotide motifs. A total of 52 alleles were detected averaging 4.7 alleles/locus. The expected heterozygosity of the eleven loci ranged between 0.368 and 0.710 and the common probability of identical genotypes was 1.8 x 107. The loci identified 27 unique profiles of the 41 Cannabis samples. The eleven microsatellite markers developed in this study were found to be useful for DNA fingerprinting and for assessing genetic relationships in Cannabis.
Identifier
FI13101576
Recommended Citation
Al-Ghanim, Hussain J., "Development of microsatellite markers in Cannabis Sativa for fingerprinting and genetic relatedness analyses" (2003). FIU Electronic Theses and Dissertations. 1091.
https://digitalcommons.fiu.edu/etd/1091
Rights Statement
In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
Comments
If you are the rightful copyright holder of this dissertation or thesis and wish to have it removed from the Open Access Collection, please submit a request to dcc@fiu.edu and include clear identification of the work, preferably with URL.