Document Type

Dissertation

Degree

Doctor of Philosophy (PhD)

Major/Program

Chemistry

First Advisor's Name

Francisco Fernandez-Lima

First Advisor's Committee Title

Committee Chair

Second Advisor's Name

Anthony DeCaprio

Second Advisor's Committee Title

Committee Member

Third Advisor's Name

Piero Gardinali

Third Advisor's Committee Title

Committee Member

Fourth Advisor's Name

Fernando Noriega

Fourth Advisor's Committee Title

Committee Member

Fifth Advisor's Name

David Chatfield

Fifth Advisor's Committee Title

Committee Member

Keywords

Analytical Chemistry, Mass Spectrometry, Trapped Ion Mobility Spectrometry, Liquid Chromatography, Biomarkers, Endocrine Disruptors, Lipids

Date of Defense

3-22-2018

Abstract

The complexity of biological matrices makes the detection and quantification of compounds of interest challenging. For successful targeted or untargeted identification of compounds within a biological environment, the use of complementary separation techniques is routinely required; in many situations, a single analytical technique is not sufficient. In the present dissertation, a multidimensional analytical technique was developed and evaluated, a combination of new sample preparation/extraction protocols, liquid chromatography, trapped ion mobility and mass spectrometry (e.g., LC-TIMS-MS and LC-TIMS-MS/MS). The performance of these techniques was evaluated for the detection of polybrominated diphenyl ethers metabolites, polychlorinated biphenyls metabolites in human plasma, opioid metabolites in human urine, and lipids in Dictyostelium discoideum cells. The new workflows and methods described in the body of this dissertation allows for rapid, selective, sensitive and high-resolution detection of biomarkers in biological matrices with increased confidence, sensitivity and shorter sample preparation and analysis time.

Identifier

FIDC006592

Available for download on Friday, October 26, 2018

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