Document Type

Thesis

Degree

Master of Science (MS)

Department

Biology

First Advisor's Name

David N. Kuhh

First Advisor's Committee Title

Committee Chair

Second Advisor's Name

John Makemson

Third Advisor's Name

Jeff Elhai

Date of Defense

12-7-1996

Abstract

Intra and Inter Vegetative Compatibility Group (VCG) heterokaryon formation was observed in Fusariurn oxysporum f.sp. cubense (Foc). Using the double-pick method to force heterokaryon formation via hyphal anastomosis, 104 pairwise combinations were done between 100 auxotrophic mutants of Foe representing races 1, 2 and 4 in five VCGs. Inter formae speciales heterokaryon formation was also observed on pairings between benomyl resistant Wild Type Testers (WTT) and wild type isolates in 15 VCGs and four different formae speciales. Microconidia analysis of heterokaryons identified both parental phenotypes as well as diploid phenotypes. Colonies with altered and hybrid genotypes were recovered from analysis of sectors from prototrophic hybrids as well as sectors with parental phenotype providing evidence of all stages of the parasexual cycle in this asexual, soilborne, phytopathogen.

Identifier

FI14061524

Comments

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Biology Commons

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