Title

Ovarian Cancer therapy combining the modalities of hyperthermia and chemotherapy

Document Type

Thesis

Degree

Master of Science (MS)

Department

Biomedical Engineering

First Advisor's Name

Anthony I. McGoron

First Advisor's Committee Title

Committee Chair

Second Advisor's Name

Chenzhong Li

Third Advisor's Name

Eric T. Grumpier

Date of Defense

4-5-2007

Abstract

Ovarian is the leading cause of deaths in women diagnosed with gynecological malignancies. Current treatments include chemotherapy which may cause severe side effects. The purpose of this study was to investigate the combinational therapeutic effect of chemotherapy and hyperthermia to an ovarian cancer cell line and compare the effects of inducing hyperthermia systemically with localized hyperthermia. Cells were seeded in 96-well plates and subjected to different doses of chemotherapy agent; Doxorubicin (DOX). To simulate systemic hyperthermia the 96-well plate was incubated for 60 minutes at 43°C. To simulate localized hyperthermia, Indocyanine Green was added to the seeded cells in 96-well plates, after which the wells were irradiated by 808 nm NIR laser. SRB assay was used at the end of the study to measure cell proliferation. The results showed that cell proliferation was reduced 110% when DOX was combined with localized hyperthermia compared with DOX or systemic hyperthermia alone.

Identifier

FI14060820

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