Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients
Document Type
Thesis
Degree
Master of Science (MS)
Major/Program
Please see currently inactive department below.
Major/Program
Medical Laboratory Science
First Advisor's Name
Janet Lineback
First Advisor's Committee Title
Committee Chair
Second Advisor's Name
Bernard Steele
Third Advisor's Name
Patrick Shen
Date of Defense
11-18-1998
Abstract
The purpose of this study was to establish a polymerase chain reaction (PCR)-restriction enzyme assay for detecting the hereditary hemochromatosis (HHC) mutation, C282Y, in gestational and gestational diabetic subjects in South Florida. DNA samples from 43 gestational subjects were amplified by PCR, digested with RsaI, and analyzed by electrophoresis. An allelic frequency of 2.33%, or 4.65% heterozygosity, was observed. The assay is successful and applicable to future studies on HHC and gestational diabetes.
Identifier
FI14060153
Recommended Citation
Cheat Eng, Lim, "Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients" (1998). FIU Electronic Theses and Dissertations. 2121.
https://digitalcommons.fiu.edu/etd/2121
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