Document Type



Master of Science (MS)


Forensic Science

First Advisor's Name

Anthony P. DeCaprio

First Advisor's Committee Title

Committee Chair

Second Advisor's Name

Jose R. Almirall

Second Advisor's Committee Title

Committee Member

Third Advisor's Name

Bruce McCord

Third Advisor's Committee Title

Committee Member


Novel Psychoactive Substances, Synthetic Cannabinoids, Oral Fluid, Liquid Chromatography, Triple Quadrupole, Mass Spectrometry, Online SPE

Date of Defense



According to NIDA, synthetic cannabinoids (SC) include man-made, mind-altering chemicals. Their typically rapid metabolism and instability in biological matrices requires fast analytical methods for detection and confirmation; therefore, the use of oral fluid (OF) matrix is advantageous because collection is fast and non-invasive. The use of liquid chromatography triple quadrupole mass spectrometry (LC-QqQ-MS) and online solid phase extraction (SPE) for sample pretreatment minimizes bias and facilitates low level detection.

This study demonstrates the first use of online SPE for sample pretreatment in the comprehensive analysis of 100 SC and metabolites in OF by LC-QqQ-MS. The method was validated according the ASB Standard 036, First Edition 2018. Results show that the quantitative method is able to meet the required criteria. Bias and precision values were



Files over 15MB may be slow to open. For best results, right-click and select "Save as..."



Rights Statement

Rights Statement

In Copyright. URI:
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).