Surface plasmon resonance experiments

Document Type



Master of Science (MS)



First Advisor's Name

Yesim Darici

First Advisor's Committee Title

Committee Chair

Second Advisor's Name

Jiandi Zhang

Third Advisor's Name

Wenzhi Li

Fourth Advisor's Name

Berrin Tansel

Fifth Advisor's Name

Xuewen Wang

Date of Defense



In this study, a Surface Plasmon Resonance (SPR) optical sensor, based on Kretschmann configuration, was designed and constructed. SPR is a useful tool for obtaining quantitative kinetic and affinity information on biomolecular interactions.

The resolution and the sensitivity of our homemade SPR system was determined and optimized by using thiols and sucrose solutions. The data were normalized and processed to increase the resolution of the system. The sensograms were obtained and the kinetic constants were analyzed. The sensogram of the thiols solutions illustrated the process of association of the thiols on gold surface. The sensogram of the sucrose solutions shown, that the sucrose doesn't have association on gold surface.



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