Surface plasmon resonance experiments
Master of Science (MS)
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In this study, a Surface Plasmon Resonance (SPR) optical sensor, based on Kretschmann configuration, was designed and constructed. SPR is a useful tool for obtaining quantitative kinetic and affinity information on biomolecular interactions.
The resolution and the sensitivity of our homemade SPR system was determined and optimized by using thiols and sucrose solutions. The data were normalized and processed to increase the resolution of the system. The sensograms were obtained and the kinetic constants were analyzed. The sensogram of the thiols solutions illustrated the process of association of the thiols on gold surface. The sensogram of the sucrose solutions shown, that the sucrose doesn't have association on gold surface.
Freixas, Angel Luis, "Surface plasmon resonance experiments" (2007). FIU Electronic Theses and Dissertations. 3417.
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