Document Type



Master of Science (MS)



First Advisor's Name

David N. Kuhh

First Advisor's Committee Title

Committee Chair

Second Advisor's Name

John Makemson

Third Advisor's Name

Jeff Elhai

Date of Defense



Intra and Inter Vegetative Compatibility Group (VCG) heterokaryon formation was observed in Fusariurn oxysporum f.sp. cubense (Foc). Using the double-pick method to force heterokaryon formation via hyphal anastomosis, 104 pairwise combinations were done between 100 auxotrophic mutants of Foe representing races 1, 2 and 4 in five VCGs. Inter formae speciales heterokaryon formation was also observed on pairings between benomyl resistant Wild Type Testers (WTT) and wild type isolates in 15 VCGs and four different formae speciales. Microconidia analysis of heterokaryons identified both parental phenotypes as well as diploid phenotypes. Colonies with altered and hybrid genotypes were recovered from analysis of sectors from prototrophic hybrids as well as sectors with parental phenotype providing evidence of all stages of the parasexual cycle in this asexual, soilborne, phytopathogen.




If you are the rightful copyright holder of this dissertation or thesis and wish to have it removed from the Open Access Collection, please submit a request to and include clear identification of the work, preferably with URL.

Included in

Biology Commons



Rights Statement

Rights Statement

In Copyright. URI:
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).