Document Type



Master of Science (MS)


Environmental Engineering

First Advisor's Name

Dr. Shonali Laha

First Advisor's Committee Title

Committee member

Second Advisor's Name

Dr. Yelena Katsenovich

Second Advisor's Committee Title

Committee member

Third Advisor's Name

Dr. Berrin Tansel

Third Advisor's Committee Title

Committee member

Fourth Advisor's Name

Dr. Michael Sukp

Fourth Advisor's Committee Title

Committee member


environmental, remediation, microorganisms, uranium, anaerobic, facultative, bacteria, chemistry

Date of Defense



Uranium (U) is a key contaminant at the Hanford site. The formation of uranyl-phosphate bearing minerals such as autunite as a result of tripoliphosphate injections has been used as a U immobilization strategy. Bacteria are known as key factors governing the fate and transport of soil contaminants. This research evaluated the interaction of facultative bacteria Shewanella Oneidensis MR-1 with autunite mineral in bicarbonate-amended media solutions. The concentration of several elements such as U, calcium (Ca) and phosphorous (P) released as a result of autunite mineral biodissolution were determined as a function of time; changes in cell density and protein assay were performed to evaluate cells viability. Results suggested that higher bicarbonate concentrations increased aqueous U, Ca and P concentrations while also allowing cells to withstand U toxicity and, additionally suggested the possibility of secondary minerals formation. This research provides a better understanding on the stability of uranyl phosphate minerals in the presence of facultative bacteria in bicarbonate-amended media solutions.





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