Title
A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons
Date of this Version
1-1-2018
Document Type
Article
Rights
default
Abstract
Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.
DOI
10.1007/978-1-4939-7459-7_18
Identifier
29177747
Recommended Citation
Annamalai T., Cheng B., Keswani N., Tse-Dinh YC. (2018) A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons. In: Drolet M. (eds) DNA Topoisomerases. Methods in Molecular Biology, vol 1703. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7459-7_18
