Document Type

Thesis

Degree

Master of Science (MS)

Department

Biomedical Engineering

Advisor's Name

Sharan Ramaswamy

Advisor's Title

Committee Chair

Advisor's Name

Anuradha Godavarty

Advisor's Name

Yei-Chih Huang

Keywords

vascular cells, periodontal ligament derived stem cells, cell migration, magnetic resonance imaging, bioreactor

Date of Defense

11-8-2011

Abstract

The design of a tissue engineered pulmonary valve (TEPV) involves cells source(s), scaffold, in vitro conditioning system and the functional stability of the TEPV in vivo. Vascular cells (pulmonary artery smooth muscle (SMCs) and endothelial cells (ECs)) and periodontal ligament derived stem cells (PDLSCs) are relevant sources for the designing of TEPVs. In this study, labeling of these cell populations with super paramagnetic iron oxide microparticles along with concomitant usage of transfection agents was followed by visualization using magnetic resonance, while Intracellular iron oxide was confirmed by prussian blue staining and fluorescence microscopy. Also, the potential of PDLSC as a feasible source for TEPVs was investigated, expressing differentiative capacity to both SMC and EC phenotypes by a combination of biochemical and mechanical stimulation. Flow conditioning in a u-shaped bioreactor augmented collagen production in SMC-EC (99.5% for n=3) and PDLSC (93.3% for n=3) seeded scaffolds after a 3-week culturing period (P<0.05).

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