Document Type
Thesis
Degree
Master of Science (MS)
Department
Biomedical Engineering
Advisor's Name
Sharan Ramaswamy
Advisor's Title
Committee Chair
Advisor's Name
Anuradha Godavarty
Advisor's Name
Yei-Chih Huang
Keywords
vascular cells, periodontal ligament derived stem cells, cell migration, magnetic resonance imaging, bioreactor
Date of Defense
11-8-2011
Abstract
The design of a tissue engineered pulmonary valve (TEPV) involves cells source(s), scaffold, in vitro conditioning system and the functional stability of the TEPV in vivo. Vascular cells (pulmonary artery smooth muscle (SMCs) and endothelial cells (ECs)) and periodontal ligament derived stem cells (PDLSCs) are relevant sources for the designing of TEPVs. In this study, labeling of these cell populations with super paramagnetic iron oxide microparticles along with concomitant usage of transfection agents was followed by visualization using magnetic resonance, while Intracellular iron oxide was confirmed by prussian blue staining and fluorescence microscopy. Also, the potential of PDLSC as a feasible source for TEPVs was investigated, expressing differentiative capacity to both SMC and EC phenotypes by a combination of biochemical and mechanical stimulation. Flow conditioning in a u-shaped bioreactor augmented collagen production in SMC-EC (99.5% for n=3) and PDLSC (93.3% for n=3) seeded scaffolds after a 3-week culturing period (P<0.05).
Recommended Citation
Martinez, Catalina, "The Effects of Dynamic Culturing Environments on Cell Populations Relevant to Heart Valve Tissue Engineering" (2011). FIU Electronic Theses and Dissertations. Paper 505.
http://digitalcommons.fiu.edu/etd/505