Master of Science (MS)
First Advisor's Name
Eric T. Crumpler
First Advisor's Committee Title
Second Advisor's Name
Nikolaos M. Tsoukias
Third Advisor's Name
Richard T. Schoephoerster
Date of Defense
Bacteria biohazards, such as Anthrax, are responsible for causing mild to serious illnesses in humans and animals. The primary aim of this research study was to develop a rapid one step assay to detect and neutralize bacteria-based biohazards, using an Immunoliposome-nanoparticle complex. An Anthrax model, Bacillus cereus, was grown for 3 hours and diluted 1:25 in media (2.0 x 107 cfu/ml). The Bacillus cereus was interacted with an Immunoliposome-nanoparticle complex containing an MgO-C 2 neutralization agent. The samples were analyzed via flow cytometry with a 1:8:1 ratio Bacillus cereus, Immunoliposome-nanoparticle complex, and Ethidium homodimer-1 for two hours. The results obtained showed when the Immunoliposome-nanoparticle complex were interacted with bacteria both detection and neutralization occurred immediately. As incubation time increased, fluorescence shifted closer to the control region. Therefore, bacteria can be immediately detected with the Immunoliposomenanoparticles complex, and high levels of neutralization can be achieved less than two hours of incubation.
De Zayas, Rosaura, "Detection and neutralization of bacterial biohazards via nanoencapsulation" (2006). FIU Electronic Theses and Dissertations. 2757.
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