Document Type

Thesis

Degree

Master of Science (MS)

Department

Biomedical Engineering

First Advisor's Name

Eric T. Crumpler

First Advisor's Committee Title

Committee Chair

Second Advisor's Name

Nikolaos M. Tsoukias

Third Advisor's Name

Richard T. Schoephoerster

Date of Defense

8-18-2006

Abstract

Bacteria biohazards, such as Anthrax, are responsible for causing mild to serious illnesses in humans and animals. The primary aim of this research study was to develop a rapid one step assay to detect and neutralize bacteria-based biohazards, using an Immunoliposome-nanoparticle complex. An Anthrax model, Bacillus cereus, was grown for 3 hours and diluted 1:25 in media (2.0 x 107 cfu/ml). The Bacillus cereus was interacted with an Immunoliposome-nanoparticle complex containing an MgO-C 2 neutralization agent. The samples were analyzed via flow cytometry with a 1:8:1 ratio Bacillus cereus, Immunoliposome-nanoparticle complex, and Ethidium homodimer-1 for two hours. The results obtained showed when the Immunoliposome-nanoparticle complex were interacted with bacteria both detection and neutralization occurred immediately. As incubation time increased, fluorescence shifted closer to the control region. Therefore, bacteria can be immediately detected with the Immunoliposomenanoparticles complex, and high levels of neutralization can be achieved less than two hours of incubation.

Identifier

FI14062227

Comments

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