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Document Type
Dissertation
Degree
Doctor of Philosophy
Department
Chemistry
Advisor's Name
José R. Almirall,
Advisor's Title
Committee Chair
Advisor's Name
Watson Lees
Advisor's Name
Bruce McCord
Advisor's Name
Yong Cai
Advisor's Name
De Etta Kay Mills
Date of Defense
11-14-2008
Abstract
Cannabis sativa is the most frequently used of all illicit drugs in the United States. Cannabis has been used throughout history for its stems in the production of hemp fiber, for its seed for oil and food, and for its buds and leaves as a psychoactive drug. Short tandem repeats (STRs), were chosen as molecular markers because of their distinct advantages over other genetic methods. STRs are co-dominant, can be standardized such that reproducibility between laboratories can be easily achieved, have a high discrimination power and can be multiplexed. In this study, six STR markers previously described for Cannabis were multiplexed into one reaction. The multiplex reaction was able to individualize 98 Cannabis samples (14 hemp and 84 marijuana, authenticated as originating from 33 of the 50 United States) and detect 29 alleles averaging 4.8 alleles per loci. The data did not relate the samples from the same state to each other. This is the first study to report a single reaction six-plex and apply it to the analysis of almost 100 Cannabis samples of known geographic collection site.
Recommended Citation
Mendoza Baez, Maria Angelica, "Genetic Individualization of Cannabis sativa by a Short Tandem Repeat Multiplex System" (2008). FIU Electronic Theses and Dissertations. Paper 270.
http://digitalcommons.fiu.edu/etd/270