Title

Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients

Document Type

Thesis

Degree

Master of Science (MS)

Department

Please see currently inactive department below.

Department

Medical Laboratory Science

First Advisor's Name

Janet Lineback

First Advisor's Committee Title

Committee Chair

Second Advisor's Name

Bernard Steele

Third Advisor's Name

Patrick Shen

Date of Defense

11-18-1998

Abstract

The purpose of this study was to establish a polymerase chain reaction (PCR)-restriction enzyme assay for detecting the hereditary hemochromatosis (HHC) mutation, C282Y, in gestational and gestational diabetic subjects in South Florida. DNA samples from 43 gestational subjects were amplified by PCR, digested with RsaI, and analyzed by electrophoresis. An allelic frequency of 2.33%, or 4.65% heterozygosity, was observed. The assay is successful and applicable to future studies on HHC and gestational diabetes.

Identifier

FI14060153

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