Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients

Cheat Eng Lim, Florida International University

Abstract

The purpose of this study was to establish a polymerase chain reaction (PCR)-restriction enzyme assay for detecting the hereditary hemochromatosis (HHC) mutation, C282Y, in gestational and gestational diabetic subjects in South Florida. DNA samples from 43 gestational subjects were amplified by PCR, digested with RsaI, and analyzed by electrophoresis. An allelic frequency of 2.33%, or 4.65% heterozygosity, was observed. The assay is successful and applicable to future studies on HHC and gestational diabetes. ^

Subject Area

Biology, Molecular|Biology, Genetics|Health Sciences, Medicine and Surgery

Recommended Citation

Cheat Eng Lim, "Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients" (January 1, 1998). ProQuest ETD Collection for FIU. Paper AAI1392488.
http://digitalcommons.fiu.edu/dissertations/AAI1392488

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